{"id":1277,"date":"2018-02-07T07:04:29","date_gmt":"2018-02-07T07:04:29","guid":{"rendered":"http:\/\/www.ptfos.unios.hr\/novicjfst\/?p=1277"},"modified":"2018-02-07T07:07:26","modified_gmt":"2018-02-07T07:07:26","slug":"1277","status":"publish","type":"post","link":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/2018\/02\/07\/1277\/","title":{"rendered":"CJFST.2018.10.1.06"},"content":{"rendered":"

\"\"<\/p>\n

Original scientific paper\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0 \u00a0 \u00a0 \u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 \u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 DOI: 10.17508\/CJFST.2018.10.1.06<\/em><\/p>\n

Glucose oxidase activity and hydrogen peroxide accumulation in Croatian honeys<\/strong><\/h1>\n

\"\"<\/a>IVICA STRELEC1*<\/sup>, BILJANA CREVAR1<\/sup>, TIHOMIR KOVA\u010c1<\/sup>, BLANKA BILI\u0106 RAJS1<\/sup>, LJILJANA PRIMORAC1<\/sup>, IVANA FLANJAK1<\/sup><\/strong><\/p>\n

1<\/sup><\/em>Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuha\u010da 20, 31 000 Osijek, Croatia<\/em><\/p>\n\n\n\n\n\n
ARTICLE INFO<\/strong><\/td>\nABSTRACT<\/strong><\/td>\n<\/tr>\n
\n

Article history:<\/em><\/strong>
\nReceived: August 24, 2017
\nAccepted: January 11, 2018<\/p>\n<\/td>\n

\n

Ability of black locust (n = 7), chestnut (n = 3), lime (n = 5), mint (n = 3), and honeydew (n = 12) honeys to produce hydrogen peroxide upon dilution, as well as their glucose oxidase activity were investigated in the present study. The glucose oxidase (GOX) activity was determined by the standard horseradish peroxidase\/o<\/em>-dianisidin method, while hydrogen peroxide concentration in honey solutions of five different mass to volume ratios (1:1; 1:2; 1:4; 1:8; 1:16) by semi-quantitative method using MQuant\u2122 peroxide test strips. The obtained results showed that chestnut, lime, honeydew and mint honeys exhibited high GOX activity (341.26 \u00b1 128.78, 350.16 \u00b1 124.91, 376.82 \u00b1 69.02, 402.47 \u00b1 60.99 \u00b5g H2<\/sub>O2<\/sub>\/h g), while black locust honeys much lower GOX activity of 25.58 \u00b1 21.87 \u00b5g H2<\/sub>O2<\/sub>\/h g. The accumulation of hydrogen peroxide in serially diluted honeys has shown asymmetrical inverted U-shaped curve, where the increase in hydrogen peroxide accumulation with dilution reached a maximum point, after which its concentration rapidly declined. Hydrogen peroxide content in honey solutions of different mass to volume ratio varied from 0 to 294.1 \u00b5mol\/L h. Lime and chestnut honeys generated the highest hydrogen peroxide content (264.71 \u00b1 65.77, 245.10 \u00b1 84.90 \u00b5mol\/L h) on average, while black locust, mint and honeydew honeys at least two-fold lower amounts (113.40 \u00b1 50.84, 127.45 \u00b1 33.96, 112.75 \u00b1 98.42 \u00b5mol\/L h).
\nLack of correlation between glucose oxidase activity and hydrogen peroxide content indicates that the glucose oxidase activity does not present a reliable parameter for the prediction of hydrogen peroxide content produced in honey solutions.<\/p>\n<\/td>\n<\/tr>\n

\n

Keywords<\/em>:<\/strong>
\nhoney,
\nhydrogen peroxide accumulation,
\nglucose oxidase activity<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Original scientific paper\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0 \u00a0 \u00a0 \u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 \u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 DOI: 10.17508\/CJFST.2018.10.1.06 Glucose oxidase activity and hydrogen peroxide accumulation in Croatian honeys IVICA STRELEC1*, BILJANA CREVAR1, TIHOMIR KOVA\u010c1, BLANKA BILI\u0106 RAJS1, LJILJANA PRIMORAC1, IVANA FLANJAK1 … Op\u0161irnije<\/a><\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1277","post","type-post","status-publish","format-standard","hentry","category-nekategorizirano"],"_links":{"self":[{"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/1277","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/comments?post=1277"}],"version-history":[{"count":2,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/1277\/revisions"}],"predecessor-version":[{"id":1279,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/1277\/revisions\/1279"}],"wp:attachment":[{"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/media?parent=1277"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/categories?post=1277"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/tags?post=1277"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}