{"id":4723,"date":"2023-05-08T08:16:54","date_gmt":"2023-05-08T08:16:54","guid":{"rendered":"http:\/\/www.ptfos.unios.hr\/novicjfst\/?p=4723"},"modified":"2023-05-08T08:21:11","modified_gmt":"2023-05-08T08:21:11","slug":"cjfst-2023-15-1-10","status":"publish","type":"post","link":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/2023\/05\/08\/cjfst-2023-15-1-10\/","title":{"rendered":"CJFST.2023.15.1.10"},"content":{"rendered":"

\"\"<\/a>Original scientific paper \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0\u00a0 \u00a0 \u00a0\u00a0 DOI: 10.17508\/CJFST.2023.15.1.10
\n<\/em><\/p>\n

Impact of extraction method on alkaloid content and antioxidant activity of Matricaria pubescens<\/em><\/strong><\/h1>\n

\"https:\/\/orcid.org\/0000-0001-7086-5147\"<\/a><\/strong>Hassiba Metrouh-Amir1*<\/sup>, Nadir Amir2,3<\/sup><\/strong><\/p>\n

1<\/sup><\/em>Laboratoire de Biotechnologies V\u00e9g\u00e9tales et Ethnobotanique, Facult\u00e9 des Sciences de la Nature et de la Vie Universit\u00e9 de Bejaia, Route de Targa-Ouzemour, 06000 Bejaia, Algeri
\n<\/em>2<\/sup><\/em>Laboratoire de maitrise des \u00e9nergies renouvelables,\u00a0\u00e9quipe biomasse et environnement, facult\u00e9 de technologie universit\u00e9 de Bejaia,\u00a0Route de Targa-Ouzemour, 06000 Bejaia, Algeria
\n<\/em>3<\/sup><\/em>D\u00e9partement de Microbiologie, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Route de Targa-Ouzemour, 06000 Bejaia, Algeria<\/em><\/p>\n\n\n\n\n\n
ARTICLE INFO<\/strong><\/td>\nABSTRACT<\/strong><\/td>\n<\/tr>\n
Article history:
\n<\/em><\/strong>Received: January 23, 2023
\nAccepted: January 31, 2023<\/td>\n		Matricaria pubescens<\/em> is a medicinal plant much appreciated by the people of the Saharan regions and widely used to treat many diseases. The present study was devoted to evaluate the effect of extraction technique, pH and nature of solvent on alkaloid content and antioxidant activity of M. pubescens.<\/em> Alkaloid extraction was carried out by applying two techniques, maceration extraction and Soxhlet extraction, using several solvents at different pH (alkaline chloroform, acid methanol, neutral methanol and neutral ethanol). In the present study, the antioxidant activity of the alkaloid extracts of M. pubescens<\/em> was evaluated using ferric reducing power, molybdate reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity, \u03b2-carotene bleaching assay, hydroxyl radical scavenging activity (assessed by deoxyribose assay and HOSC assay) and oxygen radical absorbance capacity (ORAC). The obtained results show that the extraction rates obtained and the antioxidant activities of alkaloid extracts were affected by the extraction technique, pH and the solvent used. The highest extraction rates were obtained with the ethanolic neutral (Soxhlet) and methanolic acidified (maceration) extracts. The alkaloid extracts of M. pubescens<\/em> exerted a reducing activity of ferric and molybdate ions. They also showed significant radical scavenger activity against DPPH, ABTS, hydroxyl, and peroxyl radicals. This significant wealth of alkaloids and these various antioxidant activities could justify the traditional use of M. pubescens.<\/em><\/td>\n<\/tr>\n
Keywords:
\n<\/em><\/strong>Matricaria pubescens
\n<\/em>alkaloids
\nantioxidant activity
\nextraction technique
\nsolvent<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Original scientific paper \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 … Op\u0161irnije<\/a><\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4723","post","type-post","status-publish","format-standard","hentry","category-nekategorizirano"],"_links":{"self":[{"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/4723","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/comments?post=4723"}],"version-history":[{"count":3,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/4723\/revisions"}],"predecessor-version":[{"id":4727,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/4723\/revisions\/4727"}],"wp:attachment":[{"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/media?parent=4723"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/categories?post=4723"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/tags?post=4723"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}