{"id":5513,"date":"2024-12-25T13:39:48","date_gmt":"2024-12-25T13:39:48","guid":{"rendered":"https:\/\/www.ptfos.unios.hr\/novicjfst\/?p=5513"},"modified":"2025-01-09T08:07:05","modified_gmt":"2025-01-09T08:07:05","slug":"cjfst-2024-16-2-04","status":"publish","type":"post","link":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/2024\/12\/25\/cjfst-2024-16-2-04\/","title":{"rendered":"CJFST.2024.16.2.04"},"content":{"rendered":"

\"\"<\/a><\/em><\/h1>\n

Study of the effect of ultrasonic-assisted extraction based on the Box-Behnken design on the phenolic compound content of Hammada scoparia<\/em> leaves and evaluation of their antioxidant and antibacterial activities<\/strong><\/h1>\n

Meriam Salemi1<\/sup>,\"https:\/\/orcid.org\/0000-0001-7086-5147\"<\/a>Hassiba Metrouh-Amir2<\/sup>*, Nadir Amir3,4<\/sup>, Hamza Moussa5<\/sup><\/strong><\/p>\n

1<\/sup><\/em>Universit\u00e9 de Bejaia, Facult\u00e9 des Sciences de la Nature et de la Vie, Laboratoire de Biotechnologies V\u00e9g\u00e9tales et Ethnobotanique, 06000 Bejaia, Algeria
\n<\/em>2<\/sup><\/em>Universit\u00e9 de Bejaia, Facult\u00e9 des Sciences de la Nature et de la Vie, Laboratoire de Biochimie Appliqu\u00e9e,
\n06000 Bejaia, Algeria
\n<\/em>3<\/sup><\/em>Universit\u00e9 de Bejaia, Laboratoire de maitrise des \u00e9nergies renouvelables, \u00e9quipe biomasse et environnement, facult\u00e9 de technologie, 06000 Bejaia, Algeria
\n<\/em>4<\/sup><\/em>Universit\u00e9 de Bejaia, Facult\u00e9 des Sciences de la Nature et de la Vie, D\u00e9partement de Microbiologie,
\n06000 Bejaia, Algeria
\n<\/em>5<\/sup><\/em>Universit\u00e9 de Bouira, Facult\u00e9 des Sciences de la Nature et de la vie et sciences de la terre, D\u00e9partement de Biologie, 10000 Bouira, Algeria<\/em><\/p>\n\n\n\n\n\n\n\n\n\n\n
ARTICLE INFO<\/strong><\/td>\nABSTRACT<\/strong><\/td>\n<\/tr>\n
TYPE: <\/strong>Original scientific paper<\/em>
\nhttps:\/\/doi.org\/10.17508\/CJFST.2024.16.2.04<\/a>
\nPDF<\/strong><\/a><\/td>\n		Hammada scoparia <\/em>is a plant of the Chenopodiaceae family. It represents a potential source of polyphenols and is used in traditional medicine in many countries. This research was carried out to evaluate the influence of ultrasound-assisted extraction parameters (ethanol concentration (30-70%), temperature (20-60 \u00b0C), time (10-30 min), and solvent-to-solid ratio (30-60 v\/w) on total phenolic compound and total flavonoid contents, as well as the antioxidant capacity (DPPH assay) of H. scoparia, <\/em>using the response surface methodology (RSM) approach and a Box-Behnken design (BBD). The obtained optimal extract of H. scoparia<\/em> was used to evaluate ferric and molybdate reducing powers and ABTS scavenging activity as well as the antibacterial activity. Based on the BBD results, the extraction conditions, which gave the highest contents of phenolic compounds and flavonoids and the greatest DPPH activity, are: an extraction time of 30 minutes, a temperature of 20 \u00b0C, an ethanol concentration of 46.95%, and a liquid-to-solid ratio of 60 mL\/g. The results of the determination of antioxidant activity revealed that H. scoparia <\/em>extract inhibited free radicals DPPH\u02d9 and ABTS\u02d9, and reduced ferric and molybdate through different mechanisms. Concerning the antibacterial activity, the results obtained showed that H. scoparia <\/em>extract was found to be active against all tested bacterial strains, with the exception of Escherichia coli<\/em> ATCC 25922. H. scoparia <\/em>optimal extract could be a promising source for the pharmaceutical industry and food applications.<\/td>\n<\/tr>\n
*<\/strong>CORRESPONDENCE
\n<\/strong>Hassiba Metrouh-Amir
\nhassiba.metrouh@univ-bejaia.dz<\/td>\n<\/tr>\n
ARTICLE HISTORY
\n<\/strong>Received: October 3, 2024
\nAccepted: November 19, 2024<\/td>\n<\/tr>\n
CITATION
\n<\/strong>Salemi M, Metrouh-Amir H, Amir N, Moussa H. Study of the effect of ultrasonic-assisted extraction based on the Box-Behnken design on the phenolic compound content of Hammada scoparia<\/em> leaves and evaluation of their antioxidant and antibacterial activities. Croatian Journal of Food Science and Technology (2024) 16 (2)\u00a0 218 – 235<\/td>\n<\/tr>\n
\"\"<\/a><\/strong>C<\/strong>OPYRIGHT: <\/strong>\u00a9 2024 by the author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)<\/em><\/a>.<\/em><\/td>\n<\/tr>\n
KEYWORDS<\/strong><\/p>\n

Hammada scoparia<\/em>; ultrasound assisted extraction; phenolic compounds; antioxidant activity; antibacterial activity; response surface methodology<\/p>\n<\/td>\n<\/tr>\n

KEY CONTRIBUTION<\/strong><\/p>\n