{"id":6516,"date":"2026-07-06T06:37:07","date_gmt":"2026-07-06T06:37:07","guid":{"rendered":"https:\/\/www.ptfos.unios.hr\/novicjfst\/?p=6516"},"modified":"2026-07-06T07:04:47","modified_gmt":"2026-07-06T07:04:47","slug":"cjfst-2026-18-1-09","status":"publish","type":"post","link":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/2026\/07\/06\/cjfst-2026-18-1-09\/","title":{"rendered":"CJFST.2026.18.1.09"},"content":{"rendered":"

\"\"<\/a><\/h1>\n

<\/h1>\n

<\/h1>\n

Pharmaceutical biology and bioactive properties of Rhamnus <\/em>alaternus<\/em> L.: A comprehensive evaluation of extraction methods and antioxidant potential<\/h1>\n

\"https:\/\/orcid.org\/0009-0004-9055-4302\"<\/strong>\u00a0Abdelkader Hamiani1,2<\/sup>, \"https:\/\/orcid.org\/0000-0002-2725-9798\"<\/strong>Salim Bouchentouf3,4*<\/sup>, \"https:\/\/orcid.org\/0009-0004-3975-6494\"<\/strong>Naima Hammoudi1<\/sup>, \"https:\/\/orcid.org\/0000-0002-2808-8847\"<\/strong>Nadia Kambouche2 <\/sup><\/p>\n

1<\/sup>University of Abdelhamid Ibn Badis, Faculty of Exact Sciences and Computer Sciences, Department of Chemistry, <\/em>Mostaganem, Algeria <\/em>
\n2<\/sup>University of Oran 1 Ahmed Ben Bella, Faculty of Exact and Applied Sciences, Laboratory of Organic Synthesis, Department <\/em>of Chemistry, Oran, Algeria <\/em>
\n3<\/sup>University of Saida (Dr. Moulay Tahar), Faculty of Sciences and Technology, Department of Process Engineering, BP 138, <\/em>Cit\u00e9 EN-NASR, Sa\u00efda 20000, Algeria <\/em>
\n4<\/sup>University of Abou Bekr Belka\u00efd, Faculty of Sciences, Department of Chemistry, Laboratory of Natural and Bioactive <\/em>Substances (LASNABIO), P.O. Box 119, Tlemcen 13000, Algeria<\/em><\/p>\n\n\n\n\n\n\n\n\n\n\n
ARTICLE INFO<\/strong><\/td>\nABSTRACT<\/strong><\/td>\n<\/tr>\n
TYPE: <\/strong>Original scientific paper
\nhttps:\/\/doi.org\/10.17508\/CJFST.2026.18.1.09<\/a>
\nPDF<\/strong><\/a><\/td>\n		Rhamnus alaternus<\/em> L. (Rhamnaceae) is used in Algerian traditional medicine for treating hepatic and dermatological conditions. This study provides a comprehensive evaluation of the phytochemical profile and bioactivities of Soxhlet extracts (dichloromethane, ethyl acetate, methanol) and hydrodistilled essential oil (1.9% yield) from Algerian R. alaternus<\/em> leaves. The methanolic extract demonstrated superior performance, exhibiting the highest total phenolic content (220.0 \u00b1 0.335 mg GAE\/g) and the most potent antioxidant activity, with a DPPH IC\u2085\u2080 of 3.50 \u00b1 0.10 \u00b5g\/mL and a FRAP value of 0.100 \u00b1 0.138 mmol Fe\u00b2\u207a\/g. Notably, this phenolic content is approximately 25% higher than that reported from other Mediterranean regions. HPLC analysis identified quercetin and ascorbic acid as the major bioactive constituents. The extracts also showed significant, broad-spectrum antimicrobial activity against foodborne pathogens, including Escherichia coli<\/em>, Pseudomonas aeruginosa<\/em>, Bacillus cereus<\/em>, and Candida albicans<\/em>, with the dichloromethane extract exhibiting antifungal effects. This study establishes the Algerian variant of R. alaternus<\/em> as a particularly rich source of natural antioxidants and antimicrobial agents, thereby validating its traditional use and highlighting its strong potential as a dual-function agent for applications in food preservation and nutraceuticals.<\/td>\n<\/tr>\n
*<\/strong>CORRESPONDENCE
\n<\/strong>Salim Bouchentouf
\nbouchentouf.salim@yahoo.fr<\/td>\n<\/tr>\n
ARTICLE HISTORY
\n<\/strong>Received: November 26, 2025
\nAccepted: April 22, 2026<\/td>\n<\/tr>\n
CITATION
\n<\/strong>Hamiani A, Bouchentouf S, Hammoudi N, Kambouche N. Pharmaceutical biology and bioactive properties of Rhamnus alaternus L.: A comprehensive evaluation of extraction methods and antioxidant potential. Croatian Journal of Food Science and Technology (2026) 18 (1) 113\u2015133<\/td>\n<\/tr>\n
\"\"<\/a><\/strong>C<\/strong>OPYRIGHT: <\/strong>\u00a9 2026 by the author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND)<\/a>.<\/td>\n<\/tr>\n
KEYWORDS<\/strong><\/p>\n

Rhamnus alaternus; natural antioxidants; antimicrobial activity; phenolic compounds; natural food preservatives<\/p>\n<\/td>\n<\/tr>\n

KEY CONTRIBUTION<\/strong><\/p>\n

Algerian Rhamnus alaternus possesses a superior phenolic profile, with its methanolic extract yielding 220.0 mg GAE\/g. It exhibits exceptional antioxidant potency, evidenced by a DPPH IC50 of 3.50 \u03bcg mL-1, comparable or superior to pure quercetin
\nand ascorbic acid. The extracts show broad-spectrum antimicrobial activity against key foodborne pathogens like E. coli<\/em>, P. aeruginosa<\/em>, and B. cereus<\/em>. Potent natural antifungal activity is revealed, with the dichloromethane extract outperforming amoxicillin against C. albicans<\/em>. These findings position R. alaternus<\/em> as a highly promising dual-function natural agent for food preservation, capable of inhibiting both oxidative rancidity and microbial spoilage.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Pharmaceutical biology and bioactive properties of Rhamnus alaternus L.: A comprehensive evaluation of extraction methods and antioxidant potential \u00a0Abdelkader Hamiani1,2, Salim Bouchentouf3,4*, Naima Hammoudi1, Nadia Kambouche2 1University of Abdelhamid Ibn Badis, Faculty of Exact Sciences and Computer Sciences, Department of Chemistry, Mostaganem, Algeria 2University of Oran 1 Ahmed Ben Bella, Faculty of Exact and Applied … Op\u0161irnije<\/a><\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-6516","post","type-post","status-publish","format-standard","hentry","category-nekategorizirano"],"_links":{"self":[{"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/6516","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/comments?post=6516"}],"version-history":[{"count":5,"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/6516\/revisions"}],"predecessor-version":[{"id":6527,"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/posts\/6516\/revisions\/6527"}],"wp:attachment":[{"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/media?parent=6516"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/categories?post=6516"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ptfos.unios.hr\/novicjfst\/index.php\/wp-json\/wp\/v2\/tags?post=6516"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}