Original scientific paper DOI: 10.17508/CJFST.2023.15.2.03
Standardization of 3,5-dinitrosalicylic acid (DNS) assay for measuring xylanase activity: detecting and solving problems
Marko Božinović1, Tea Sokač2,
Anita Šalić1*, Ana-Marija Dukarić3, Marina Tišma3, Mirela Planinić3, Bruno Zelić1,4
1University of Zagreb, Faculty of Chemical Engineering and Technology, Marulićev trg 19, 10000 Zagreb, Croatia
2University of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10000 Zagreb, Croatia
3Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, F. Kuhača 18, 31 000 Osijek, Croatia
4University North, Trg dr. Žarka Dolinara 1, HR-48000 Koprivnica, Croatia
| ARTICLE INFO | ABSTRACT |
| Article history: Received: December 2, 2022 Accepted: March 27, 2023 |
The 3,5-dinitrosalicylic acid (DNS) assay has been used for many years mainly to determine the enzymatic activity of xylanase. The assay is based on the detection of reduced sugars. Although the method is widely used, several recent studies have questioned the accuracy of the method. They mainly focused on the detection of side reactions that could lead to a false positive result of the assay. In this study, the basic components of the DNS assay such as buffer preparation, substrate source and concentration, incubation time, reagent preparation, and activity calculation were re-evaluated. Potential problems were detected and a new assay procedure was proposed. Compared to literature data, the new assay is shorter because it avoids the generation of a calibration curve and takes into account the enzyme and substrate amount when calculating enzyme activity, which is neglected in current assays. |
| Keywords: xylanase enzyme activity DNS assay spectrophotometric assay |