Determination of sildenafil mixed into herbal honey mixture by ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry

Neira Mustabasic1*, Selin Isik2

 1Ankara University, Institute of Forensic Science, Department of Forensic Chemistry and Forensic Toxicology, Cebeci Research and Application Hospital,
06590 Dikimevi, Ankara, Turkey
Near East University, Faculty of Pharmacy, Department of Analytical Chemistry, 99138 Nicosia, Turkish Republic of Northern Cyprus

original scientific paper
DOI: 10.17508/CJFST.2017.9.2.13


There has been a number of reports of natural products contaminated with illegal adulterants that threaten consumer health because of their adverse pharmacological effects worldwide. In this study, a multi-residual ultra-performance liquid chromatography method with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was applied for the identification of sildenafil added into a herbal honey mixture used as an immune system booster. Electrospray ionization (ESI) source was applied and operated in the positive ion mode. The mobile phase consisted of 0.1% formic acid aqueous solution/acetonitrile (70:30, v/v) using the isocratic gradient elution system at a detection wavelength of 290 nm. The compound of sildenafil added into traditional herbal mixed honey was identified according to the spectrum, chromatographic behavior, and mass spectral data were identified by comparison with the reference substance. The method is selective, sensitive and can be used to detect the sildenafil illegally added into traditional herbal medicinal preparations.

Keywords: ultra-performance liquid chromatography, quadrupole time-of-flight mass spectrometry, honey, sildenafil, herbal products