CJFST.2020.12.1.15

Original scientific paper                                                                                                                                                      DOI: 10.17508/CJFST.2020.12.1.15

Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan

NOSHEEN NAZ1, AMBREEN NAZ2*, ALLAH RUKHA1, SHABBIR AHMAD2, UMMARA WAHEED3, KASHIF RAZZAQ4, UMAR FAROOQ2 AND NIGHAT RAZA2

1National Institute of Food Science and Technology, Faculty of Food, Nutrition and Home Sciences, University of Agriculture, Faisalabad, Pakistan
2Department of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan
3Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan
4Department of Horticulture, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan

ARTICLE INFO ABSTRACT
Article history:
Received: January 1, 2018
Accepted: February 26, 2020
The current research was planned to measure the comparative phytochemical and anti-oxidative potential of aqueous and methanolic extracts of two indigenously grown millet varieties of Pakistan. The locally available millet varieties, i.e. MB-87 and AF-POP flours were chemically characterized through the proximate and mineral analysis. The antioxidant extract was prepared in water and methanol. The extracts were screened for saponins, terpenoids, flavonoids and tannins; methanolic extract of MB-87 and AF-POP showed flavonoids in an average quantity (++), whilst aqueous extract had only trace (+) values. The phytochemical screening showed the presence of saponins only in the aqueous extract of both varieties. However, terpenoids and tannins were present in both methanolic and aqueous extracts. During antioxidant evaluation, millet methanolic extracts showed higher level of TPC and DPPH of MB-87 and AF-POP as 0.30±0.001 & 0.23±0.0012 mg GAE/g and 48±0.96 &46±1.09%, respectively. However, the β-carotene activity of the aqueous extract of both varieties (MB-87 and AF-POP) was recorded higher. In a nutshell, the methanolic extract of MB-87 has an appreciable atioxidant profile. Furher research should be planned to screen the important constituents of Pakistani millet varieties.
Keywords:
millet
phytochemicals
antioxidant
MB-87
AF-POP