Electrochemical characterization and detection of vitamin E in real samples
Dominik Goman*, Valentina Danković, Olivera Galović, Martina Medvidović-Kosanović
Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Cara Hadrijana 8/A, 31000 Osijek, Croatia
| ARTICLE INFO | ABSTRACT |
| TYPE: Original scientific paper https://doi.org/10.17508/CJFST.2024.16.1.01 |
Vitamin E is an important antioxidant and plays an essential role in cellular respiration. At room temperature, it exists in the form of viscous oil and is highly soluble in fat. Vitamin E is a group of eight compounds: four tocopherols and four tocotrienols. In this study, α-tocopherol (α -TOH) was characterized using cyclic and differential pulse voltammetry and was detected with high-performance liquid chromatography in real samples (kale, parsley, and a powdered dietary supplement). Cyclic and differential pulse voltammograms have shown one oxidation peak which corresponds to the oxidation of α -TOH. In the oxidation of α -TOH, rapid two-electron oxidation coupled with one proton loss (–2e−/–1H+) occurred, and a diamagnetic cation (α -TO+) was formed. Oxidation of α -TOH α is an irreversible and a diffusion-controlled process since the linear correlation between anodic peak current, Ip,a, and the square root of scan rate (ν)1/2 was observed. It was also determined that the anodic peak current of α -TOH increases with the increase of its concentration (linear response was obtained in the concentration range from 0.43 mg/dm3 to 70.41 mg/dm3). The obtained values of α -TOH in real samples were 8.267 mg/kg for parsley, 1.492 mg/kg for kale, and 0.067 mg/kg for powdered dietary supplement. |
| *CORRESPONDENCE Dominik Goman gomandominik@gmail.com |
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| ARTICLE HISTORY Received: December 19, 2023 Accepted: January 12, 2024 |
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| CITATION Goman D, Danković V, Galović O, Medvidović-Kosanović M. Electrochemical characterization and detection of vitamin E in real samples. Croatian Journal of Food Science and Technology (2024) 16 (1) xx-xx |
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 COPYRIGHT: © 2024 by the author(s). This is an open-access article distributedunder the terms of the Creative Commons Attribution License (CC BY). |
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| KEYWORDS
α –tocopherol; cyclic voltammetry; differential pulse voltammetry; high-performance liquid chromatography; vitamin E |
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| KEY CONTRIBUTION
Characterization of α -tocopherol using cyclic and differential pulse voltammetry. Combination of electrochemical methods and high performance liquid chromatography for the characterization and detection of vitamin E. Oxidation mechanism of α -tocopherol. |