Chemical composition and biological potential of garlic by-products

Andrea Tadić¹, Danijela Skroza¹*, Martina Čagalj², Roberta Frleta Matas³, Maja Veršić Bratinčević⁴, Vida Šimat²

¹University of Split, Faculty of Chemistry and Technology, Department of Food Technology and Biotechnology, Ruđera Boškovića 35, 21000 Split, Croatia
²University of Split, Department of Marine Studies, Ruđera Boškovića 37, 21000 Split, Croatia
³University of Split, Faculty of Science, Center of Excellence for Science and Technology-Integration of Mediterranean Region (STIM), Ruđera Boškovića 35, 21000 Split, Croatia
⁴Institute for Adriatic Crops and Karst Reclamation, Department of Applied Science, Put Duilova 11, 21000 Split

ARTICLE INFO	ABSTRACT
TYPE: Original scientific paper https://doi.org/10.17508/CJFST.2025.17.2.04 PDF	Garlic is a vegetable from the Allium genus that has been valued for its medicinal properties since ancient times. Today, alongside traditional white garlic, increasing attention is being given to black garlic (produced through the thermal processing of white garlic at a specific temperature and relative humidity), a food with equally beneficial properties and rich nutritional value that is associated with numerous health benefits and a valuable nutritional composition. During the thermal processing of garlic, valuable by-products are formed, characterized by a high content of phenolic compounds, which are known for their exceptional biological properties, particularly their antioxidant and antimicrobial activity. The aim of this research was to determine and compare the chemical composition and biological potential of extracts from white and black garlic by-products. Extracts were prepared using microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE). The total phenolic content (TPC) of the extracts was determined using the Folin-Ciocalteu method, while specific phenolic compounds were identified using the HPLC method. The antioxidant potential was evaluated using FRAP, ORAC, and DPPH methods, and the antimicrobial activity was assessed by determining minimal inhibitory and bactericidal concentration. The results indicate a high TPC, with rutin identified as the dominant flavonoid, and a good biological potential for all tested extracts. Extracts from black and white garlic by-products exhibited similar antimicrobial activity against all tested microorganisms, with better activity observed against Gram-positive species, particularly Listeria monocytogenes. The extract from white garlic by-products obtained through microwave-assisted extraction demonstrated the highest antioxidant activity as measured by the DPPH (43.38%) and ORAC (39.02 mM TE/L) methods, along with the highest total phenolic content (60.10 mg GAE/g), indicating its exceptional potential for application in the food industry as a natural alternative to synthetic additives.
*CORRESPONDENCE Danijela Skroza danci@ktf-split.hr
ARTICLE HISTORY Received: May 8, 2025 Accepted: June 26, 2025
CITATION Tadić A, Skroza D, Čagalj M, Frleta Matas R, Veršić Bratinčević M, Šimat V. Chemical composition and biological potential of garlic by-products. Croatian Journal of Food Science and Technology (2025) 17 (2) 1 – 10
COPYRIGHT: © 2025 by the author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
	KEYWORDS garlic; black garlic; by-product; phenolics; HPLC; antioxidant activity
	KEY CONTRIBUTION The chemical composition and biological potential of by-products from white and black garlic were investigated. The extracts were prepared using microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE). The highest total phenolic content and antioxidant were obtained by MAE. The extracts showed strong antimicrobial activity, especially against Listeria monocytogenes. Extracts from white garlic by-products showed potential as natural additives in the food industry.